■ 基本信息

■ 质粒属性

■ 质粒简介

pCAMBIA1391是一个植物启动子检测报告载体，可通过MCS克隆进需要研究的启动子。  

The pCambia vector backbone is derived from the pPZP vectors. The pCambia1391 vector offers:

High copy number in E.coli for high DNA yields  pVS1 replicon for high stability in Agrobacterium

Small size  Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest

Bacterial selection with kanamycin

Plant selection with hygromycin B

Simple means to construct translational fusions to gusA reporter genes

Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins. Designed to utilize gusA as a true reporter of gene expression by fusion construction this vector contains a promoterless, non-intron gusA gene that retains the initiation codon of the NcoI site. This permits simple construction of carboxy-terminus protein fusions to gusA.

■ 质粒图谱

■ 质粒序列

质粒序列请下载：ZK1712 pCAMBIA1391植物表达质粒.txt