■ 基本信息
别名: | pET51b |
启动子: | T7 |
复制子: | pBR322 |
终止子: | T7 terminator |
质粒分类: | 大肠杆菌载体；PET系列表达质粒 |
质粒大小: | 5218bp |
质粒标签: | N-Strep-Tag II，N-EK，C-10*His |
原核抗性: | Amp |
克隆菌株: | DH5a |
培养条件: | 37℃，有氧，LB |
表达宿主: | BL21(DE3) |
诱导方式: | IPTG或乳糖及其类似物 |
5'测序引物: | T7:TAATACGACTCACTATAGGG |
3'测序引物: | T7-ter:TGCTAGTTATTGCTCAGCGG |
■ 质粒属性
质粒宿主: | 大肠杆菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF
|
片段物种: | 空载体
|
原核抗性: | Amp |
真核抗性: |
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荧光标记: |
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■ 质粒简介
pET-51b是一个大肠杆菌pET系列表达质粒。The pET-51b(+) vector carries an N-terminal Strep•Tag II coding sequence (1) followed by a rec-ognition site for enterokinase, and an optional C-terminal His•Tag coding sequence. The presence of two "gentle elution" tags at both the N-terminus and C-terminus is ideal for dual purification strate-gies designed to isolate full-length fusion proteins (2). Unique restriction sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below the circle map. The f1 origin is oriented so that infection with the helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
■ 质粒图谱
■ 质粒序列
质粒序列请下载：
ZK190pET-51b大肠表达质粒.txt
